发明名称 |
ANALYSIS OF INTRACELLULAR MODIFICATIONS |
摘要 |
<p>Improved methods of determining the intracellular state of a protein as well as modifications of the protein are provided by introducing a surrogate fusion protein comprising a member of an enzyme fragment complementation complex and a target protein. After exposing cells transformed with the surrogate fusion protein to a change in environment, e.g. a candidate drug, the cells are lysed, the lysate separated into fractions or bands, conveniently by gel electrophoresis and transferring the proteins by Western blot to a membrane. The bands on the membrane are developed using the other member of the enzyme fragment complementation complex and a substrate providing a detectable signal. The method is found to provide high sensitivity and the ability to observe modifications of the target protein.</p> |
申请公布号 |
EP1766397(A4) |
申请公布日期 |
2009.02.25 |
申请号 |
EP20050764161 |
申请日期 |
2005.06.29 |
申请人 |
DISCOVERX, INC. |
发明人 |
HORECKA, JOSEPH;FUNG, PETER;EGLEN, RICHARD, M. |
分类号 |
G01N33/53;C12Q1/02;G01N33/535;G01N33/566 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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