| 摘要 |
FIELD: chemistry. ^ SUBSTANCE: invention relates to production of highly-purified highly-active plasminogen-activating enzyme preparations, which may be used in biochemistry and medical science. Method implies the following: Morikrasa enzyme preparation is dissolved in tris-chloride buffer with pH 7.2-7.5 to obtain concentration of at least 2.5%. Resulting solution is fractionated by means of ion-exchange chromatography on DEAE-Toyoperl sorbent. Proteinase is eluted with NaCl concentration gradient of 0.1-1.5 M. Then, resulting solution of plasminogen activator is concentrated and demineralised by ultrafiltration using a membrane permeable for peptides with molecular weight up to 10 kDa. Plasminogen activator is purified by gel chromatography on Sephadex G 50 in 0.1 M ammonium-acetate buffer with pH 6.3-6.5, which contains 1.0-1.2 M of sodium chloride. ^ EFFECT: simplified method of production and shorter process time. ^ 3 dwg, 1 tbl |
