发明名称 |
METHOD OF SEPARATION OF DEOXYRIBONUCLEIC ACIDS |
摘要 |
The present invention relates to a method of separating nucleic acids from a liquid, which comprises providing a mobile phase comprising nucleic acids and a first salt, which forms lyotropic ions when dissolved; passing said mobile phase across a hydrophobic interaction chromatography (HIC) matrix to adsorb deoxyribonucleic acid(s); passing an eluent across said matrix to desorb one or more deoxyribonucleic acid(s), which eluent comprises the first salt as well as an increasing gradient provided by a second salt, which second salt forms less lyotropic ions when dissolved than the first salt; and isolating at least one fraction comprising separated deoxyribonucleic acid(s).
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申请公布号 |
US2009047734(A1) |
申请公布日期 |
2009.02.19 |
申请号 |
US20070282668 |
申请日期 |
2007.03.29 |
申请人 |
GE HEALTHCARE BIO-SCIENCES AB |
发明人 |
BERG MIKAEL;BLOM HANS;LEMMENS RAF |
分类号 |
B01D15/08;C07H21/00;C12N15/64 |
主分类号 |
B01D15/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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