发明名称 METHOD FOR DETERMINING THE VIABILITY OF CELLS IN CELL CULTURES
摘要 The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently colored sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.
申请公布号 EP2024727(A1) 申请公布日期 2009.02.18
申请号 EP20070724498 申请日期 2007.04.24
申请人 SARTORIUS STEDIM BIOTECH GMBH 发明人 OBERMANN, STEFAN;BAUMFALK, REINHARD;REIF, OSCAR-WERNER;WURM, FLORIAN;DE JESUS, MARIA;STETTLER, MATTHIEU;JORDAN, MARTIN
分类号 G01N15/04;G01N33/50;G01N33/58 主分类号 G01N15/04
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