| 摘要 |
<p>The present invention relates to a method for producing purified and/or concentrated analytes of interest from a sample of biological sources, said method comprising or consisting of the following steps:
a) providing an affinity column capable of being centrifuged, the column comprising or consisting of:
(i) a centrifugation column,
(ii) optionally one, two or more separators, and
(iii) an affinity matrix comprising a matrix and one or more, the same or different binding moieties, wherein the binding moieties are attached to the matrix in such a way, that the binding moieties are capable of binding to the analytes of interest to form a binding moiety-analyte-complex so that the analytes of interest are not eluted from the column,
b) optionally washing the affinity column with a washing solvent,
c) adding the sample comprising the analytes of interest to and filtering the sample through the affinity column, so that at least part of the analytes of interest of the sample form the binding moiety-analyte-complex,
d) optionally washing the affinity column comprising the binding moiety-analyte-complexes with a washing solvent,
e) adding an incubating solvent to said affinity column comprising the binding moiety-analyte-complexes,
f) incubating the incubation solvent on the affinity column comprising the binding moiety-analyte-complexes, so that at least part of the binding moiety-analyte-complexes are destroyed and
g) eluting the analytes of interest from the affinity column by way centrifugation,
a use of an affinity column capable for being centrifuged, a kit comprising an affinity column and an affinity column.</p> |
