TRANSIENTLY BONDING DRAG-TAGS FOR SEPARATION MODALITIES
摘要
<p>The invention relates transiently attaching drag-tags to molecules during electrophoresis. The invention includes running buffers having drag-tags that transiently attach to lipophilic moieties attached to the molecules. The lipophilic moieties can be covalently or ionically bonded to the molecules. One particular aspect of the invention is a nucleoside analog or a nucleic acid analog comprising a lipophilic moiety. The invention is also directed to methods of separating molecules that comprise a lipophilic moiety. The methods generally comprise transiently attaching a drag-tag to the lipophilic moiety during a separation modality. These methods can be used to separate the molecules by size or weight, to measure a hydrodynamic radius of a drag-tag, or to separate a plurality of drag-tag by their hydrodynamic radius.</p>
申请公布号
WO2009020710(A2)
申请公布日期
2009.02.12
申请号
WO2008US67856
申请日期
2008.06.23
申请人
CARNEGIE MELLON UNIVERSITY;SCHNEIDER, JAMES, W.;GROSSER, SHANE, T.;SAVARD, JEFFREY, M.
发明人
SCHNEIDER, JAMES, W.;GROSSER, SHANE, T.;SAVARD, JEFFREY, M.