| 摘要 |
An object of the present invention is to provide a hydrophilic polymer microparticle which shows reduced swelling in an aqueous medium and has an excellent dispersibility in an aqueous medium, a filler for ion-exchange liquid chromatography which can effectively suppress non-specific adsorption of protein and the like, a method for analyzing glycosylated hemoglobin using the filler for ion-exchange liquid chromatography, a method for production of a filler for ion-exchange liquid chromatography that can maintain suppressive effects on swelling, non-specific adsorption and the like for a long period of time, a filler for ion-exchange liquid chromatography produced by the method for production of a filler for ion-exchange liquid chromatography, and a filler for ion-exchange liquid chromatography for glycosylated hemoglobin analysis. The present is a hydrophilic polymer microparticle, which has a DW/DA ratio of 2.0 or less, the DW representing a particle diameter of the hydrophilic polymer microparticle dispersed in water, the DA representing a particle diameter of the hydrophilic polymer microparticle dispersed in acetone, each of the particle diameters measured by a particle size distribution analyzer after the hydrophilic polymer microparticle is dispersed in water or acetone, subjected to irradiation with ultrasonic waves for 15 minutes, and allowed to stand at a temperature of 25° C. for 240 hours for equilibration; and has a contact angle with water of 70° or less, the contact angle measured using a contact angle measurement apparatus at a temperature of 25° C. by forming a droplet of pure water on a single layer of the hydrophilic polymer microparticles arranged with no space therebetween.
|
