| 摘要 |
PROBLEM TO BE SOLVED: To provide a method capable of simply aggregating magnetic particles having a surface modified with a thermoresponsive polymer at a given temperature without heating or cooling an aqueous solution containing an adsorbent and a sample, and to provide a separation method and a detection method of a substance to be detected in the sample using the method. SOLUTION: The method of separating a substance to be detected from a sample includes the steps of: mixing the adsorbent and the sample in the aqueous solution to adsorb the substance to be detected on the adsorbent, and aggregating the adsorbent by changing a salt concentration in the aqueous solution; and collecting the adsorbent from the aqueous solution by a magnetic force, wherein the adsorbent comprises a magnetic particle of an average particle size of 50 to 1000 nm, a surface of which is modified with a thermoresponsive polymer and is immobilized with a substance having an affinity for the substance to be detected. COPYRIGHT: (C)2009,JPO&INPIT |
