TEMPERATURE REGULATED GENE EXPRESSION

摘要

A method for regulation of gene expression by variation of temperature uses a riboswitch. The riboswitch includes a 5'-UTR construct of crhC which alters its secondary structure in response to temperature, resulting in a more stable transcript at lower temperatures, permitting translation. At higher temperatures, the transcript is destabilized and functionally inactive. The 5'-UTR construct of crhC may be operatively linked to a promoter and a gene and administered to cells with an expression vector.