摘要 |
[Objective] To provide a biosensor chip which is capable of stably immobilizing bioactive substances, as well as preventing non specific adsorption of substances which are the targets of detection. [Constitution] A biosensor chip is constituted by: a substrate; a metal film which is physically attached to the substrate; and a self assembled monolayer constituted by alkyl chains, which is physically attached to the metal film. Ligands and at least one functional group, selected from a group consisting of a hydroxy group, a carboxyl group, an alkoxy group, a methyl group, and hydrogen atoms are physically attached to the self assembled monolayer. The ratio of the number of alkyl chains which are covalently bonded to the ligands with respect to the total number of alkyl chains on the substrate is within a range of 0.40 to 0.99. |