| 摘要 |
<p>Live poliomyelitis virus and/or antigenic material derived therefrom is purified and/or concentrated by adsorbing the live virus and/or antigenic material at a pH of less than 7 from an aqueous medium on to aluminium phosphate and subsequently eluting the live virus and/or antigenic material therefrom by means of an aqueous medium at a pH greater than 7. The pH for adsorption depends upon the virus type and quantity of aluminium phosphate used. For Type I virus, a pH of less than 5.8 and preferably 4.7 to 5.2 is used. For Types II and III a pH of less than 6.8 and preferably 5.0 to 5.5 is used. In general the pH should be at least 4.5 for absorption. The aluminium phosphate is used in concentrations of 0.05-8 mg/ml. and preferably 0.1 to 4 mg/ml. Elution is preferably at pH 8 to 11. Suitable eluants are aqueous solutions of alkali metal and alkaline earth metal hydroxides, carbonates and bicarbonates and aqueous solutions of disodium or dipotassium hydrogen phosphate. Process may be applied to the virus-containing liquid after the virus has been inactivated. Vaccines are produced from the material so formed.</p> |
