发明名称 |
ENZYMATIC SYNTHESIS OF SULFATED POLYSACCHARIDES WITHOUT IDURONIC ACID RESIDUES |
摘要 |
Iduronic acid (IdoUA)-free heparan sulfate (HS)-like compounds are provided. Also provided are methods of synthesizing IdoUA-free HS-like compounds. The methods can include providing at least one O-sulfotransferase (OST) enzyme and a reaction mixture comprising 3'-phosphoadenosine 5'-phosphosulfate (PAPS); and incubating a polysaccharide substrate with the at least one OST and the reaction mixture, whereby the HS-like compounds are synthesized. Also disclosed are methods of synthesizing a library of HS-like compounds and methods of determining the mechanism of activity of HS-like compounds.
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申请公布号 |
US2009035787(A1) |
申请公布日期 |
2009.02.05 |
申请号 |
US20080178434 |
申请日期 |
2008.07.23 |
申请人 |
THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
发明人 |
LIU JIAN;JONES COURTNEY;CHEN JINGHUA;XU YONGMEI |
分类号 |
G01N33/53;C08B37/10;C12P19/26;C12Q1/02;C40B50/06 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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