| 摘要 |
<p>Provided are a skin cell co-culture method of mixing and culturing keratinocyte, melanocyte, fibroblast, and endothelial cell which are extracted from minimal donor sites and a cell therapeutic composition using the same. Since various types of skin cells extracted from one type of donor site are mixed and co-cultured, due to a paracrine effect between cells, all the cells can be well grown even in a serum-free medium. In addition, the cells can be well grown in a medium that is not a medium specified for culture of each type of cells. In addition, the cells can be well grown even in a case where the number of cells is too small to be well grown in a separate culture. In addition, unlike the separate culture where freezing storage and thawing of melanocyte is almost not available, the mixed co-cultured cells can be well grown after the freezing storage and thawing. In addition, expression of an SMA (smooth muscle actin) which is induced in an in-vitro fibroblast culture is not induced in the mixed co-culture, so that fibroblast can be cultured without induction of differentiation. A cell therapeutic including the mixed, co-cultured skin cells can show an effective wound healing capability in comparison with conventional cell therapeutics including only the keratinocyte.</p> |
