IMMUNOSTIMULANTS INCLUDING THE LYTIC EXTRACT OF CANDIDA SAKE

摘要

An immunostimulant containing the lystic extract of Candida sake is provided to increase activity of neutrophil and lymphocyte as major immune cells of animals, thereby protecting animals from diseases. The immunostimulant contains the lystic extract of Candida sake which is prepared by cultivating Candida sake in yeast malt extract agar broth at 30 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Candida sake suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.