Process for preparing an in ovo combination viral vaccine

摘要

A process for the preparation of a viral vaccine, the process comprising culturing at least two different viruses in an embryonated avian egg. The process preferably consists of inoculating the egg with a first virus and subsequently inoculating the egg with additional viruses, the inoculation further preferably takes place 8 to 14 days after fertilization of the egg. The different viruses are preferably Newcastle disease virus (NDV) and infectious bursal disease virus (IBDV), and are further preferably the La Sota, B1 or lentogenic strains of NDV and the VMG 91 strain of IBDV. Also claimed is a stabilising solution comprising 0.5 to 10% w/w polyvinylpyrrolidone, 1 to 30% w/w bactopeptone and 0.5 to 10% w/w sodium glutamate.