| 摘要 |
A method of preserving sperm of domestic animals especially porcine and bovine. Cryopreserved boar spermatozoa is much less fertile than that of other species, which could be due to damage to sperm membrane lipids during the cryopreservation process. Incorporation of selected lipids improves the survival of boar spermatozoa following cryopreservation. Liposomes were made from lipids extracted from head plasma membrane (HPM) of boar spermatozoa or from selected lipids (SL) which contained specific phospholipids and fatty acyl chains. SL and HPM improved sperm viability (SYBR-14 and propidium iodide) a nd motility during cooling to 5.degree.C, with SL~ egg yolk better than or equal to HPM (P<0.05). Post-thaw, egg yolk showed a strong cryoprotective effect. Compared to BPM, SL-treated sperm had higher post-thaw viability, progressive motility and total motility in t he extender including egg yolk and higher viability in the extender excluding egg yolk (P<0.05).
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