| 摘要 |
This invention relates to a rapid and efficient method for carrying out enzy me- linked immunosorbent assay for detection of minute quantities of biomolecule s such as antigen, antibody etc. This invention particularly relates to microwave mediated immobilization of antigen or antibody on to the activated surface followed by performing subsequent steps of ELISA by controlled microwave irradiation. The invented procedure has dramatically reduced the total time required for ELISA to less than 10 minutes from hours to days. Th e invented ELISA procedure is rapid, economical, reproducible and simple and c an be automated. The invented procedure is useful for carrying out ELISA in clinical diagnostics, molecular biology, agriculture, sericulture, food technology, environmental science, biomedical research and other related fields.
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