发明名称 |
A METHOD FOR PRESERVATION OF HUMAN HEMATOPOIETIC STEM OR PROGENITOR CELLS |
摘要 |
Maintenance of quiescent hematopoietic stem and progenitor cells (HSPC) in culture without the addition of exogenous growth factors is an important aspect in clinical hematology. A recent report described the ability of Flt3 receptor-interacting lectin (FRIL) in the maintenance of cord blood (CB) derived progenitors in vitro. Since FRIL is a mannose binding lectin we examined the effectiveness of four such lectins of well-characterized specificities on the preservation of HSPC of CB origin. The HSPC preservation activity of lectins was assessed by in vitro colony forming unit (CFU) and long-term culture initiating cell (LTC-IC) assays. It was found that all four lectins had a HSPC preservation activity at least up to 30 days in culture without addition of exogenous growth factors. The results indicate that use of such lectins may provide a cost effective method of HSPC maintenance for clinical use. |
申请公布号 |
KR20090009186(A) |
申请公布日期 |
2009.01.22 |
申请号 |
KR20087015593 |
申请日期 |
2008.06.26 |
申请人 |
DEPARTMENT OF BIOTECHNOLOGY;INDIAN INSTITUTE OF SCIENCE;NATIONAL CENTER FOR CELL SCIENCES |
发明人 |
KALE VAIJAYANTI P.;LIMAYE LALITA S.;HINGE ASHWINI;SUROLIA AVADHESHA |
分类号 |
C12N1/08;C12N5/00;C12N5/02;C12N5/0789 |
主分类号 |
C12N1/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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