发明名称 |
Method For Site-Specifically Introducing Non-Natural Amino Acid Into Protien Using Mitochondrial Protein and Method For Effectively Preparing Trna |
摘要 |
This invention is intended to provide a protein synthesis system used for producing a tryptophan analogue-containing non-natural-amino-acid-containing protein that satisfies the following conditions: (i) tRNA that transfers a non-natural amino acid is not recognized by an endogenous aminoacyl tRNA synthetase (aaRS); (ii) it is recognized selectively by aaRS exclusive for a non-natural amino acid; and (iii) endogenous tRNA is not recognized by aaRS exclusive for a non-natural amino acid. In the eukaryotic organism-derived cell-free protein synthesis system, a yeast mitochondrial tryptophanyl tRNA synthetase is used in combination with mitochondrial tRNATrp (mt tRNATrp).
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申请公布号 |
US2009023139(A1) |
申请公布日期 |
2009.01.22 |
申请号 |
US20050791150 |
申请日期 |
2005.11.22 |
申请人 |
NISHIKAWA KAZUYA;YOKOGAWA TAKASHI;OHNO SATOSHI |
发明人 |
NISHIKAWA KAZUYA;YOKOGAWA TAKASHI;OHNO SATOSHI |
分类号 |
C12Q1/68;C07H21/04;C07K16/00;C12N9/10;C12P21/06 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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