发明名称 |
XENO-FREE CULTURE CONDITIONS FOR HUMAN EMBRYONIC STEM CELLS AND METHODS THEREOF |
摘要 |
The present disclosure provides novel culture system and methods for culturing and propagating hESCs in a substantially undifferentiated state for several passages. The ability to grow such cells without differentiation has important applications for therapeutic uses of ES cells for treating human disorders using tissue transplantation and/or gene therapy techniques. In particular, the disclosure further relates to conditioned medium obtained from human germ lineage derived feeder cells (GLDF). The hESC lines are derived, cultured and propagated in substantially undifferentiated state using the conditioned media from GLDF cells of the disclosure. In particular, the disclosure relates to the xeno-free derivation, culture and propagation of hESCs using conditioned medium of GLDF cells obtained thereof. |
申请公布号 |
WO2008075378(A3) |
申请公布日期 |
2009.01.22 |
申请号 |
WO2007IN00594 |
申请日期 |
2007.12.17 |
申请人 |
STEMPEUTICS RESEARCH PRIVATE LIMITED;TOTEY, SATISH;KULKARNI, KUMAR UDAY;SAXENA, SHOBHIT |
发明人 |
TOTEY, SATISH;KULKARNI, KUMAR UDAY;SAXENA, SHOBHIT |
分类号 |
C12N5/0735 |
主分类号 |
C12N5/0735 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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