摘要 |
<P>PROBLEM TO BE SOLVED: To provide a fluorescent protein minimally affecting a target protein. <P>SOLUTION: Dimeric and monomeric mutants of the naturally occurring tetrameric Anemonia sulcata Green Fluorescent Protein 499 (AsGFP499), peptides thereof and polynucleotides encoding them, and their use as intracellular fluorescent markers particularly in the set up of cell-based or cell-free assays, are provided, respectively. <P>COPYRIGHT: (C)2009,JPO&INPIT |