发明名称 |
Methods for purifying highly anionic proteins |
摘要 |
<p>The present invention is directed to a method for dissociating Fc containing molecules from complexes of Protein A/Fc containing molecules in a mixture comprising contacting the mixture with a metal chelate chromatographic column (IMAC) under pH conditions between 5.0 and 5.7, wherein the pH conditions are established by the addition of ethylene glycol to said mixture to dissociate Fc containing molecules from complexes of Protein A/Fc containing molecules.</p> |
申请公布号 |
EP1714982(B1) |
申请公布日期 |
2009.01.21 |
申请号 |
EP20060010184 |
申请日期 |
2002.06.05 |
申请人 |
GENETICS INSTITUTE, LLC |
发明人 |
COFFMANN, JONATHAN L.;FOSTER, WILLIAM B.;SUN, SHUJUN |
分类号 |
C07K16/00;G01N33/53;B01D15/20;B01D15/32;C07K1/20;C07K1/22;C07K1/36;C07K14/31;C07K19/00;G01N30/04;G01N30/26;G01N30/88 |
主分类号 |
C07K16/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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