发明名称 |
SPECIFIC AND SENSITIVE NUCLEIC ACID DETECTION METHOD |
摘要 |
Method for the detection of a nucleic acid comprising the production of a plurality of amplificates of a section of this nucleic acid with the aid of two primers, one of which can bind to a binding sequence A of the nucleic acid and the other can bind to a binding sequence C' which is complementary to a sequence C which is located in the 3' direction from A and does not overlap with A, contacting the amplificates with a probe having a binding sequence D which can bind to a sequence B which is located between the sequences A and C or to the complement thereof, and detecting the formation of a hybrid of the amplificate and probe where the sequence located between the binding sequences A and C contains no nucleotides that do not belong to the binding sequence D of the probe or its complement D'.
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申请公布号 |
CA2308368(C) |
申请公布日期 |
2009.01.20 |
申请号 |
CA19982308368 |
申请日期 |
1998.11.03 |
申请人 |
ROCHE DIAGNOSTICS GMBH |
发明人 |
BARTL, KNUT;HABERHAUSEN, GERD;ORUM, HENRIK;KESSLER, CHRISTOPH |
分类号 |
C12N15/09;C12Q1/68;C07H21/00;C12P19/34;C12Q1/70;C40B30/04 |
主分类号 |
C12N15/09 |
代理机构 |
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主权项 |
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地址 |
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