CARTRIDGE AND RECOMBINANT PLASMID FOR EXPRESSION AND SECRETION OF HUMAN MECHANODEPENDENT GROWTH FACTOR (MGF), STRAIN SACCHAROMYCES CEREVISIAE - MGF PRODUCER AND METHOD OF MGF PRODUCTION

摘要

FIELD: chemistry, biochemistry. ^ SUBSTANCE: invention refers to genetic engineering and bioengineering and can be used for production of recombinant human mechanodependent growth factor (MGF). Recombinant human mechanodependent growth factor is produced by cultivation of yeast cells containing plasmid which provides expression of protein secretor, and liberation of end product from culture fluid. Plasmid providing expression of human mechanodependent growth factor secretor contains expression cartridge including GAL1 gene promotor and termination region of yeast CYC1 gene transcription, pre- MFalpha1 gene region and DNA sequence, coding end protein. This plasmid is composed on the basis of plasmid pKX. Besides for production of human mechanodependent growth factor, strain Saccharomyces cerevisiae YBS618/pKX-MGF - producer of specified produced is also used. ^ EFFECT: expression of human mechanodependent growth factor secretor in yeast. ^ 6 cl, 2 dwg, 1 tbl, 7 ex