NEW POLYMERS AND THEIR USE AS FLUORESCENT LABELS

摘要

The present invention relates to a polymer composed by two to ten monomers of formula (I) as well as to a process for its preparation and its use as fluorophore. wherein: X is a radical of formula (II) wherein -R5 is an electron pair or a (C1-C3)-alkyl radical; -Ra and -Rb are radicals independently selected from the group consisting of H, (C1-C4)-alKyI, (C1-C4)-alkoxy, (C1-C4)-alkylam?no, phenyl, F, Cl, Br, amino, hydroxy, and nitro or -Ra and -Rb are fused forming with the carbon atoms to which they are attached a ring of formula (III) with the condition that (I) when -R5 is an electron pair, a is a N=C double bond, and Ra and Rb are fused forming the ring (III), said ring being a biradical selected from (IIIa) and (IIIb). thus, radical (II) is (IIa) or (IIb) respectively (II) when -R5 is a (C1-C3)-alkyl radical, ais a N-C single bond and Ra and Rb are fused forming the ring (III), said ring being a biradical (a), thus, the radical (II) is (IIc) R1-R4 and R7-R18 represent radicals, same or different, selected fro the group consisting of H1 (C1-C4)-alkyl,, (C1-C4)-alkylamipo, phenyl, F, Cl, Br, amino, hydroxy, and nitro; p is an integer from 0 to 1; R6 is a biradical selected from the group consisting of -CO-; -CONH(CH2)mCO-; and -CO[NHCHR''CO]m-, wherein -R" are side chains radicals, same or different, corresponding to natural aminoacids; and m is an integer from 1 to 3; Z is a triradical of formula (IV) wherein r is an integer from O to 1; v is an integer from O to 1; Z' is a triradical selected from -CH2- and nitrogen; Z" is H, with the proviso that: (a) when Z' is nitrogen, forming an amide bound with R6, then is hydrogen and v is an integer from O to 1, and (b) when Z" is -NH-, forming an amide group with R6, then Z1 is -CH2 - and v= O or of formula (V) wherein Z" is selected from -CH3 and -CH2NH-, Zlv is selected from H and NH, Zv is selected from S and O atom, W is an integer from 0 to 1, with the proviso that (c) when R6 is bound to Z"' then Z"' is -CH2NH-, Z?v is hydrogen and w is 0; and (d) when Z?v is -NH- forming an amide bound with R6, Z'" is - CH3 and w is 1; and and wherein the monomers of formula (I) are linked through the triradical Z, forming an amide or phosphate bound.