Human foreskin cells suitable for culturing stem cells

摘要

The invention relates to a method of maintaining human embryonic stem cells in an undifferentiated state comprising co-culturing the human embryonic stem cells with a human foreskin feeder cell line, wherein cells of said human foreskin feeder cell line are obtained from a human foreskin tissue from an 8-30 day old male individual; are growth suppressed and are capable of maintaining the human embryonic stem cells in the undifferentiated state for at least 100 passages; and wherein said co-culturing is effected in the presence of serum or serum replacement.