发明名称 |
METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF GENE EXPRESSION BY DOUBLE-STRANDED RNA |
摘要 |
The invention provides compositions and methods for selectively reducing the expression of a gene product from a desired target gene, as well as treating diseases caused by expression of the gene. The method involves introducing into the environment of a cell an amount of a double-stranded RNA (dsRNA) such that a sufficient portion of the dsRNA can enter the cytoplasm of the cell to cause a reduction in the expression of the target gene. The dsRNA has a first oligonucleotide sequence that is between 26 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of from about 19 to about 23 nucleotides is complementary to a nucleotide sequence of the RNA produced from the target gene.
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申请公布号 |
US2009018321(A1) |
申请公布日期 |
2009.01.15 |
申请号 |
US20080137914 |
申请日期 |
2008.06.12 |
申请人 |
INTEGRATED DNA TECHNOLOGIES, INC.;CITY OF HOPE |
发明人 |
ROSSI JOHN J.;BEHLKE MARK A.;KIM DONGHO |
分类号 |
C07H21/02;A61K48/00;C12N15/11;C12N15/113;C12N15/85;C12Q1/68 |
主分类号 |
C07H21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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