| 摘要 |
The present invention provides systems and methods for normalizing data in nucleic acid amplification procedures. In general, the methods include: obtaining input from a user regarding a number of normalizer measurements to use in calculations; obtaining raw fluorescence measurements of one or more probe(s) over multiple cycles of the procedure; obtaining normalizer measurement(s); and normalizing the probe data using the normalizer measurement(s) specified by the user. The present invention also discloses software and a computer readable medium for carrying method steps of the invention. Reduced cycle times, decreased variability, and/or lower Ct values are obtained as a result of the normalization.
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