| 摘要 |
A method for the production of a Factor VIII polypeptide, the method comprising a) culturing a mammalian cell transfected with an expression vector comprising a nucleic acid molecule encoding said Factor VIII polypeptide and expression. control regions operatively linked to thereto, in a cell culture medium under conditions.suitable for expression of said polypeptide; and b) isolating the expressed Factor VIII polypeptide; wherein the cell culture medium in step a) comprises a tissue inhibitor of metalloproteinase (TIMP). |
