RECOMBINANT NORTH AMERICAN TYPE 1 PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS AND METHODS OF USE

摘要

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pr oblem in the pork industry worldwide. The inclusion of markers in vaccines w ill allow for diagnostic differentiation of vaccinated animals from those na turally infected with wild-type virus. Using a cDNA infectious clone of Nort h American Type 1 PRRSV, a recombinant green fluorescent protein (GFP) tagge d PRRSV has been made, containing deletion of an immunogenic epitope, ES4, i n the nsp2 region. GFP and ES4 epitope-based ELISAs compliment the marker id entification.