INDUCIBLE/REGULATED GENE EXPRESSION SYSTEM IN E. COLI

摘要

An expression system for transforming E. coli with a nucleic add molecule of interest has an operator sequence of a cmt operon operatively linked to a promoter for the operator, and, a repressor sequence from a cym operon operatively linked to a promoter for the repressor. The expression system may have a nucleic acid molecule of interest, for example, a nucleic acid molecule that encodes a protein. Any type of E. coil host cells may be transformed with the expression system. A method of producing a protein involves transforming an E. coil host cell with the expression system having a nucleic acid molecule that codes for a protein, and, culturing the host cell in a culture medium under conditions in which the nucleic acid molecule will express the protein.