THE USE OF PLANT GLYCOSYL HYDROLASES WITH CARBOHYDRATE BINDING MODULES TO ALTER PLANT CELL WALL COMPOSITION AND STRUCTURE, OR ENHANCE DEGRADATION

摘要

The present invention discloses a transgenic plant cell which includes a nucleic acid construct. The nucleic acid construct contains a nucleic acid molecule encoding a plant endo-l,4-ß-xylanase and/or a plant endo-l,4-ß- glucanase, where the plant endo-l,4-ß-xylanase and/or the plant endo-l,4-ß- glucanase each have a modular carbohydrate binding domain, or multiple modular carbohydrate binding domains. The nucleic acid construct also includes a plant promoter and a plant termination sequence, where the plant promoter and the plant termination sequence are operably coupled to the nucleic acid molecule and at least one of the plant promoter or the plant termination sequence is heterologous to the nucleic acid molecule. The present invention also relates to methods of producing transgenic plants, polysaccharide depolymerizing the transgenic plants and non-transgenic plants, and identifying plants capable of undergoing enhanced polysaccharide depolymerization.