SEMI-SOLID OSTEOBLAST COMPOSITION CONTAINING FIBRIN FOR BONE UNION AND METHOD PREPARING THE SAME

摘要

SEMI-SOLID OSTEOBLAST COMPOSITION CONTAINING FIBRIN FOR BONE UNION AND METHOD PREPARING THE SAME PROVIDED ARE A SEMI-SOLID OSTCOBLAST COMPOSITION CONTAINING FIBRIN FOR 5 BONE UNION AND A METHOD FOR PREPARING THE SAME. THE METHOD COMPRISES ISOLATING OSTCOBLASTS FROM A BONE TISSUE AND CULTURING/PROLIFERATING THE ISOLATED OSTEOBLASTS IN DMEM (DULBECCO'S MODIFIED EAGLE'S MEDIUM) OR A -MEM (MINIMUM ESSENTIAL MEDIUM, ALPHA MODIFICATION) TO PREPARE AN OSTEOBLAST SUSPENSION; AND MIXING THE RESULTING OSTEOBLAST SUSPENSION WITH A SEMI-SOLID 10 OSTEOBLAST COAGULATION FACTOR TO PREPARE AN OSTEOBLAST THERAPEUTIC AGENT. IN ACCORDANCE WITH THE PRESENT INVENTION HAVING THE ABOVE-MENTIONED CONSTRUCTION, IT IS POSSIBLE TO ACHIEVE BONE GRAFTING CAPABLE OF PERFORMING BONE UNION HAVING NO CLINICAL GRAFT REJECTION AND UNIFORIN DISTRIBUTION OF OSTCOBLASTS VIA INJECTION OF AN OSTCOBLASTS AND FIBRIN MIXTURE INTO AFFECTED PARTS FOR BONE UNION, AND 15 EFFECTIVE AND RAPID BONE UNION VIA CONSTANT INJECTION OF A SEMI-SOLID OSTEOBLAST COMPOSITION INTO AFFECTED PARTS. AM @J -@ O I 1@0 LOT