MARKER GENES BASED ON DOXORUBICIN TREATMENT FOR SCREENING OF DRUG INDUCING CARDIOTOXICITY AND SCREENING METHOD USING THEREOF

摘要

Marker genes for screening drug inducing cardiotoxicity and a screening method thereof are provided to monitor and determine drugs or chemical substances having the risk of heart toxicity by using the reaction genes selected through a DNA microarray chip. A screening method drug inducing cardiotoxicity comprises (1) a step for processing an object compound in HUVEC; (2) a step for separating RNA from the experimental group cell which processes the object compound of the step (1) and the control cell which does not process the object compound; (3) a step for respectively making the experimental group and control group by different fluorescent materials while synthesizing RNA of the experimental group of the step (2) and the control group to cDNA; (4) a step for mixing the cDNA respectively marked by different fluorescent materials of the step (3) with the DNA microarray; (5) a step for analyzing the DNA reacted microarray of the step (4); and (6) a step for comparing and confirming the expressed grade of the marker gene with the control group.