METHOD FOR DETERMINING POLYMORPHISMS AND FUNCTIONAL MUTATIONS OF UDP-GLUCURONOSYLTRANSFERASE 1A GENES

摘要

A method for determining polymorphisms and functional mutations of UDP-glucuronosyltransferase 1A genes is provided to be used for the genic type analysis of UGT1A family genes of Asia human race including Japanese, Korean and China. A method for determining polymorphisms and functional mutations of UDP-glucuronosyltransferase 1A genes comprises (1) a step for collecting a biological material from the human; (2) a step for extracting a nucleic acid from the sample collected from the step (1); (3) a step for amplifying human UGT1A family genes by using the nucleic acid extracted from the step (2); and (4) a step for confirming the functional variant of the UGT1A family gene selected from a group consisting of 39(TA)6>(TA)7, 211G>A, 233C>T and 686C>A in UGT1A1, 31T>C, 133C>T and 140T>C in UGT1A3, 31C>T, 142>G and 292C>T in UGT1A4, 19T>G, 541A>G and 552A>C in UGT1A6, 387T>G, 391C>A, 392G>A, 622T>C and 701T>C in UGT1A7, and -118T9>T10, 726T>G and 766G>A in UGT1A9, by analyzing the sequence of the gene amplified in the step (3).