A NOVEL DENGUE ENVELOPE DOMAIN III-BASED TETRAVALENT PROTEIN VACCINE

摘要

The present invention relates to a novel recombinant envelop domain - III based tetravalent protein having SEQ ID No.: 1 which elicits protective immune responses against each of the four serotypes of dengue virus, DEN-I, DEN-2, DEN-3 and DEN-4. The invention also relates to a novel polynucleotide sequence having SEQ ID No.: 2 which encodes novel recombinant tetravalent protein and which is codon optimized for expression in eukaryotic expression system. The process for the preparation and purification of novel recombinant tetravalent protein is also disclosed. The process involves chemically synthesizing the novel polynucleotide sequence having SEQ ID No.:2, codon optimizing the sequence, followed by cloning, transforming and purifying the novel recombinant tetravalent protein. The novel recombinant tetravalent protein of the present invention is with and without secretory signal peptide. The present invention also discloses that the novel recombinant tetravalent protein results in inhibition of infectivity of each dengue virus serotype.