THE DETECTION METHOD OF LISTERIA MONOCYTOGENES USING PCR AND ELISA,& THE DETECTION KIT

摘要

A detection method of Listeria monocytogenes as sitotoxism bacteria by using the polymerase chain reaction(PCR) and enzyme-linked immune reaction is provided to rapidly and accurately detect the Listeria monocytogenes in the field of manufacture, keeping, circulation and sale of foodstuff. The detection method of Listeria monocytogenes by using the polymerase chain reaction and enzyme-linked immune reaction comprise the steps of: performing PCR with the PCR primer which selectively detects the hly gene encoding the listeriolysin as the pathogenic factor(virulence factor) of the Listeria monocytogenes; hybridizing the PCR product with the probe which specifically binds to the PCR product; and applying the hybridized PCR product to ELISA(enzyme-linked immune reaction).