摘要 |
A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNT1-Ga1T) comprising a CTS domain of N-acetylglucosaminyl transferase (GNT1) fused to a catalytic domain of beta-1,4galactosyltransferase (Ga1T), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.
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申请人 |
MEDICAGO INC.;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;UNIVERSITE DE ROUEN |
发明人 |
D'AOUST, MARC-ANDRE;MARQUET-BLOUIN, ESTELLE;BARDOR, MURIEL;BUREL, CAROLE;FAYE, LOIC;LEROUGE, PATRICE;VEZINA, LOUIS-PHILIPPE;GOMORD, VERONIQUE;AQUIN, STEPHANIE;RIHOUEY, CHRISTOPHE;PACCALET, THOMAS;SOURROUILLE, CHRISTOPHE |