METHOD FOR CULTURING CELL POPULATION CONTAINING NK CELLS AND USE OF THE CELL POPULATION

摘要

It is intended to provide a method for efficiently proliferating and activating a cell population containing a high percentage of NK cells and ?dT cells. Further, it is also intended to provide a pharmaceutical and a therapeutic method in which the cell population and a monoclonal antibody therapeutic agent are used in combination for enhancing an effect of the monoclonal antibody therapeutic agent via ADCC. By adding glucocorticoid and IL-18 to peripheral blood mononuclear cells after initiation of culture, NK cells and ?dT cells can be selectively proliferated, and a cell population containing a large amount of NK cells and ?dT cells with a high growth rate can be obtained. The obtained cell population has an excellent cytotoxic activity and cell preservation stability. Further, the ratio of CD16-positive cells is high, therefore, an effect of enhancing ADCC is high. Accordingly, the NK cells can increase a therapeutic effect of a monoclonal antibody therapeutic agent via ADCC.