发明名称 |
VECTORS AND METHODS FOR SELECTING OPEN READING FRAMES |
摘要 |
The invention provides vectors and methods designed for screening large random DNA fragment libraries for the presence of open reading frames that are free of both internal ribosome binding sites (IRBS) and stop codons. The invention overcomes the principal limitation of known ORF-selector systems, namely the potential for ORF-induced folding interference of the downstream fused reporter, by not fusing the reporter to the ORF, but rather by providing a mechanism for coupled translation of an unfused downstream reporter.
|
申请公布号 |
WO2008051619(A3) |
申请公布日期 |
2008.12.18 |
申请号 |
WO2007US22769 |
申请日期 |
2007.10.24 |
申请人 |
LOS ALAMOS NATIONAL SECURITY, LLC;WALDO, GEOFFREY, S. |
发明人 |
WALDO, GEOFFREY, S. |
分类号 |
C40B40/02 |
主分类号 |
C40B40/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|