Immunoassays using F(AB')2 fragments

摘要

Immunoassays for antigens or haptens are effected using, instead of an immunoglobulin antibody, the F(ab')2 fragments thereof. In this way, interference from endogenous RF and Clq in the fluid under assay is avoided without the necessity of pre-treating the fluid to remove or inactivate the RF or Clq. The assays may be of various types including latex agglutination and competitive binding assays. A reagent for such use comprising F(ab')2 fragments insolubilized on a water-insoluble substrate, especially a particulate substrate, is described.