| 摘要 |
An aminopeptidase enzyme is prepared by culturing a fungus capable of producing enzymes which lyse L-leucine-p-nitranilide and L-cystine-bis-p-nitranilide (according to a specified test) and which also lyse L-leucinamide. The fungus may be an Aspergillus, e.g. A. oryzae, A. sojae, A. parasiticus, A. melleus or A. flavus; the Aspergillus may be replaced at least in part by another fungus such as Rhizopus niveus, Trichoderma koningi, or Penicillium citrium. The enzyme may be purified by heating an aqueous solution containing a cobalt salt, which deactivates any other enzymes present, followed by salting out or solvent precipitation, and chromatography for example on DEAE-cellulose.
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