发明名称 |
Immunogenic HBsAg derived from transformed yeast. |
摘要 |
<p>The surface antigen protein of human Hepatitis B virus is synthesized in Saccharomyces cerevisiae as a 23,000-26,000 dalton polypeptide, essentially free of intermolecular disulfide bonds. This antigen is a poor immunogen in animals and man. No prior precedent or method exists for efficiently converting the non-disulfide bonded antigen to a fully intermolecular disulfide bonded particle. We describe the first example of such a conversion in vitro and show that the act of this conversion enhances the immunogenicity of the antigen about 10-fold. The in vitro conversion makes practical the production of hepatitis B surface antigen from microorganisms using recombinant DNA methods.</p> |
申请公布号 |
EP0135435(A2) |
申请公布日期 |
1985.03.27 |
申请号 |
EP19840401696 |
申请日期 |
1984.08.21 |
申请人 |
MERCK & CO., INC. |
发明人 |
FRIEDMAN, ARTHUR;LEHMAN, E. DALE;MCALEER, WILLIAM J.;SCHAEFER, TED F.;SCOLNICK, EDWARD M.;WAMPLER, EUGENE D. |
分类号 |
A61K39/29;A61K39/00;C07K14/02;(IPC1-7):A61K39/29 |
主分类号 |
A61K39/29 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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