摘要 |
PURPOSE:To produce aspartase in high yield in a short cultivation time, by culturing a bacterial strain belonging to Brevibacterium genus in a medium containing a specific amount of fumaric acid. CONSTITUTION:A bacterial strain belonging to Brevibacterium genus and capable of producing aspartase is cultured in a medium containing 0.5-2.8wt%, preferably 1-2.6wt% fumaric acid, at 20-40 deg.C, preferably 25-35 deg.C and 5-10pH, preferably about 7-8pH, aerobically under aeration and agitation, or shaking, etc. |