| 摘要 |
PURPOSE:To obtain the titled vector capable of utilizing various kinds of restriction enzymes, by linking the plasmid pAMalpha1 of Streptococcus faecalis DS5 with the vector pACYC177 of Escherichia coli in a specific state. CONSTITUTION:The plasmid pAMalpha1 of Streptococcus faecalis DS5 (ATCC14508) and the vecto pACYC177 of Escherichia coli are enzymatically incised in a manner to include the tetracycline-resistant gene region (Tc) originated from pAMalpha1, ampicillin-resistant gene region (Amp), and both of the replication initiation region (Ori pAMalpha1), originated from pAMalpha1 and the replication initiation region (Ori 177) originated from pACYC177. The incised fractions are linked together inserting a poly-linker having the recognition sites of the restriction enzymes EcoRI and HindIII between the fractions. |
