MONOCLONAL ANTIBODY

摘要

PURPOSE:The titled cellular surface antigen recognition type antibody, produced by a hybridoma prepared by using a cellular strain (MKN-45) derived from a gastric low differentiation type glandular cancer as an immunization source, having a light chain of IgG2a and capable of exhibiting positive reaction to cancer cells, e.g. lung cancer, colonic cancer, gastric cancer, etc. CONSTITUTION:A monoclonal antibody obtained by fractionating a cell extract of an established cancer cell (MKN-45) derived from a gastric low differentiation type glandular cancer by gel filtration, mixing the resultant high-molecular fraction having 700,000-1,500,000 molecular weight with complete Freund's adjuvant, immunizing an animal, e.g. mouse, with the mixture, collecting a cell producing an antibody, carrying out cell fusion with a myelomatous cell of an animal of the same species, cloning the resultant cell to give a cloned hybridoma, transplanting the resultant cloned hybridoma to the abdominal cavity of an animal, e.g. BALB/C mouse, to produce the aimed antibody capable of exhibiting the positive reaction to cancer cells of lung cancer (PC-3), colonic cancer (Colo201), gastric cancer (MKN-45, or KATO-III), hepatic cancer (HEK) and nasopharyngeal cancer (KB) and recovering and purifying the antibody from the ascites.