发明名称 |
Method and apparatus for ligand detection |
摘要 |
A process and apparatus has been developed for radioassay of ligand in solution which eliminates the separation step required in conventional techniques. A chamber is provided containing a quenching solution, a plurality of ligand molecules and a plurality of receptor molecules. One of pluralities forms a free species labelled with a beta particle emitter while the other is immobilized on a solid support, e.g., the chamber wall or a microbead, within the chamber. Ligand introduced with the sample competes with ligand molecules already in the chamber for receptor sites on the receptor molecules and the free species is allowed to diffuse about the chamber. A beta particle detector in communication with the chamber at a fixed position detects only those beta particles emitted from within the quenching distance of the quenching solution. The quenching properties of the solution are used in place of the conventional separation step. The process and apparatus are easily adapted for continuous monitoring of ligand level and are particularly well suited for use in radioimmunoassays. The apparatus can be miniturized allowing implantation in an animal body and in vivo monitoring of ligand level in bodily fluids.
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申请公布号 |
US4626513(A) |
申请公布日期 |
1986.12.02 |
申请号 |
US19830550400 |
申请日期 |
1983.11.10 |
申请人 |
MASSACHUSETTS GENERAL HOSPITAL |
发明人 |
BURTON, JAMES A.;HOOP, JR., BERNARD |
分类号 |
G01N33/534;G01N33/542;(IPC1-7):G01N33/543;G01N33/544 |
主分类号 |
G01N33/534 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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