PRODUCTION OF BIOLOGICALLY ACTIVE FORMS OF CSF USING A BACULOVIRUS (AcNPV)-INSECT CELL EXPRESSION SYSTEM

摘要

The expression of biologically active CSFs in insect cells (Spodoptera frugiperda) is disclosed. Numerous forms of CSF-1 are expressed and secreted as biologically active dimers. DNA transfer vectors and expression vectors that encode forms of CSF-1 and allow for their expression are disclosed and claimed. G-CSF is additionally expressed in insect cells in active form. DNA transfer vectors and expression vectors that encode G-CSF are disclosed and claimed.