| 摘要 |
<p>PURPOSE:To efficiently propagate and cultivate crocin-containing cells in a large amount, by respectively using special culture media in steps of inducing calli from the growth point of saffron and propagating the calli. CONSTITUTION:In a step of inducting calli from the growth point of saffron, an agar solid nutrient culture medium containing kinetin in 0.5-20mg/l concentration and alpha-naphthaleneacetic acid in 5-20mg/l concentration is used. In a step of propagating the calli and propagating saffron cells, a liquid nutrient culture medium containing kinetin in 0.5-20mg/l concentration and alpha- naphthaleneacetic acid in 5-20mg/l concentration is used.</p> |
