| 摘要 |
A variety of chimeric plasmids each of which comprises (a) a tetracycline resistance gene (Tc) deriving from pAM alpha 1, one of the plasmids retained by Streptococcus faecalis, (b) an ampicillin resistance gene (Amp) deriving from pACYC177, one of the vectors applicable to Escherichia coli and (c) either or both of origins, an origin (OripAM alpha 1) deriving from the plasmid pAM alpha 1 and an origin (Ori177) deriving from the vector pACYC177, the tetracycline resistance gene existing on each of which has the unique cleavage site for the restriction enzyme BalI on the entire DNA of the same plasmid and the ampicillin resistance gene existing on each of which has the unique cleavage sites for respective restriction enzymes BglI and PstI on the entire DNA on the same plasmid. There is also provided a chimeric plasmid vector containing (a) a tetracycline resistance gene region (Tc) derived from the plasmid pAM alpha 1 of Streptococcus faecalis DS5 (ATCC14508), (b) an ampicillin resistance gene region (Amp) derived from the vector pACYC177 of E. coli, (c) a first DNA replication origin (OripAM alpha 1) derived from the plasmid pAM alpha 1, (d) a second DNA replication origin (Ori177) derived from the vector pACYC177, and (e) a polylinker region having recognition and cleavage sites for the restriction enzymes EcoRI at one terminal and HindIII at the other terminal of the polylinker DNA sequence. This is a Continuation-in-part of Ser. No. 574,180, filed Jan. 24, 1984 abandoned, and Ser. No. 737,038, filed May 22, 1985, abandoned.
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